PCR-SSCP typing of MHC in cattle and buffaloes


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Authors

  • D L PIPALIA
  • C G JOSHI
  • D N RANK
  • B P BRABMKSHTRI
  • J V SOLANKI

Keywords:

BoLA-DRB3, Polymorphism, PCR-SSCP

Abstract

BoLA-DRB3 gene polymorphism was investigated in 2 cattle breeds (Gir and Kankrej) and 2 buffalo breeds (Jaffrabadi and Mehsani) by PCR single strand conformation, Genomic DNA was isolated from 42, 45, 42 and 25 blood samples collected each from Gir, Kankrej, Jaffrabadi and Mehsani animals respectively, A 284 bp BoLA-DRB3 exon 2 segment was amplified by hemi-nesting PCR, using the locus specific primers, The PCR products were subjected to a non-denaturing gel electrophoresis on MDE gel. Out of total 16 SSCP patterns observed, Gir showed 13, Kankrej 10, Jaffrabadi 14 and Mehsani 4 different patterns.

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Author Biographies

  • D L PIPALIA
    Surya Nivas, Opp, Akshar Mandir, Gondal 360 311,

  • C G JOSHI
    Associate Research Scientist,
  • D N RANK
    Associate Research Scientist,
  • B P BRABMKSHTRI
    Associate Professor, Department of Animal Oenetics and Breedirig, College of Veterinary Science and Animal Husbandry, Sardar Krushinagar 385 506
  • J V SOLANKI
    Professor and Head, Department of Animal Genetics and Breeding, College of Veterinary Science and  Animal Husbandry, Anand.

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Issue

Vol. 74 No. 6 (2004)

Section

Articles

License

The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.

How to Cite

PIPALIA, D. L., JOSHI, C. G., RANK, D. N., BRABMKSHTRI, B. P., & SOLANKI, J. V. (2014). PCR-SSCP typing of MHC in cattle and buffaloes. The Indian Journal of Animal Sciences, 74(6). https://epubs.icar.org.in/index.php/IJAnS/article/view/38949