Detection of Aeromonas hydrophila proteins immunogenic in rabbit


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Authors

  • S Maji
  • P Mali
  • S N Joardar

Keywords:

Aeromonas hydrophila, AGPT, CIE, SDS-PAGE, Weternblot

Abstract

Isolates (12) of Aeromonas hydrophila were generated from haemorrhagic ulcerative lesi ons of goldfish, Carassius auratus L. Soluble protein antigen was prepared from one representative strain, A. hydrophila GFU 10 and was subsequently subjected to SOS-PAGE analysis. It revealed 19 polypeptide bands in the range of 19 kOa to 111 kDa. seroreactivity of the antigen was assessed in agar gel precipitation test (AGPT) and counter current immunoelectrophoresis (CIE) using hyperimmune serum raised in rabbit. Westernblot analysis showed that the antigen of the strain contained three immunogenic polypeptides of approximate molecular weight 57,23 and 21 kDa indicating their serodiagnostic potentiality.

 

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Issue

Vol. 76 No. 6 (2006)

Section

Short-Communication

License

The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.

How to Cite

Maji, S., Mali, P., & Joardar, S. N. (2011). Detection of Aeromonas hydrophila proteins immunogenic in rabbit. The Indian Journal of Animal Sciences, 76(6). https://epubs.icar.org.in/index.php/IJAnS/article/view/3904