Evaluation of ELISA and IHA for the detection of agglutinins in goats vaccinated with an experimental killed Mycoplasma mycoides subsp capri saponin vaccine


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Authors

  • RAJNEESH RANA
  • N C SRIVASTAVNA
  • V P SINGH
  • S K SRIVASTAVA

Keywords:

Agglutinin, ELISA, IHA, Mycoplasma mycoides subsp capri, Vaccine

Abstract

An indirect ELISA was used for the first time to detect Mycoplasma capri antibodies in goats and was compared with
indirect haemagglutinatioll test. Goats (36) under 1 year of age and of either sex (negative for M.capri antibodies) were used in 3 groups of 17,17 and 2. Two different freeze-dried M.capri saponin vaccines i.e. vaccine A (protein concentration 1.0 mg/dose) and vaccine B (protein concentration 1.5 mg dose) were prepared and inoculated in 2 different group of goats (17 animals in each group). Indirect haemagglutination test could detect early antibodies whereas; ELISA could detect both early and late antibodies. Enzyme linked immunosorbent assay was adjudged to be comparatively superior for the detection of M.capri agglutinins.

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Author Biographies

  • RAJNEESH RANA
    Scientist (Senior Scale), Division of Animal Health, Central Institute tor Research on Goats, Makhdoom, Farah 281 122, Uttar Pradesh.

  • N C SRIVASTAVNA
    Formerly Principal Scientist,
  • V P SINGH
    Senior Scientist,
  • S K SRIVASTAVA
    Principal Scientist and Head.

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Issue

Vol. 74 No. 8 (2004)

Section

Articles

License

The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.

How to Cite

RANA, R., SRIVASTAVNA, N. C., SINGH, V. P., & SRIVASTAVA, S. K. (2014). Evaluation of ELISA and IHA for the detection of agglutinins in goats vaccinated with an experimental killed Mycoplasma mycoides subsp capri saponin vaccine. The Indian Journal of Animal Sciences, 74(8). https://epubs.icar.org.in/index.php/IJAnS/article/view/39080