Induction of immune response in goats immunized with Brucella melitensis 16M DNA

Abstract

The direct injections of B.melifensis DNA were given by intramuscular rout at different dose rates (0.5mg, 1.0mg and
1.5 mg/animal) to 3 groups of goats, each group comprised 4 goats each. Two groups were kept as control (1 positive and
1 negative). Three injections at 3-week intervals were given. The humoral immune response was analyzed every week (up
to 5 week) and Iymphoproliferative response was analysed at 15th and 30th day after the last DNA injection. Humoral
response measured 5 weeks post inoculation indicated that 1.0mg DNA injection leads to the generation of long-lived IgG
responses. Cellular immune response induced by the DNA was analyzed by studying T-cell response. Both 0.5 long and
1.5mg DNA injection resulted in low specific T-cell proliferation in response to the Brucella melitensis extracts while 1.0
mg DNA inoculated animals showed comparatively high T-cell proliferation. This specific induced proliferative response
was also found at 30 day postinoculation.