Randomly amplified polymorphic DNA (RAPD) fingerprinting of Indian sheep breeds*


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Authors

  • K GANESH KUMAR
  • PUSHPENDRA KUMAR
  • SANJEEV KUMAR
  • T K BHATTACHARYA
  • B BHUSHAN
  • S P S AHLAWAT

Keywords:

BSF, Genetic distance, Indian sheep breeds, MAPD, RAPD-PCR

Abstract

Variation in the DNA fingerprint of Indian sheep breeds, viz. Madras Red, Mandya, Marwari and Muzaffarnagari were analyzed by RAPD-PCR technique to estimate the genetic relatedness and diversity within and between breeds. The DNA isolated by phenol extraction method was analyzed using 6 random primers, All the 6 primers amplified satisfactorily and 4 of them were used to produce polymorphic patterns. The data so generated was used to estimate band sharing frequency (BSF), mean average percentage difference (MAPD) and genetic distance among the breeds. Within breed BSF was highest in Mandya and between breed BSF was highest between Mandya and Madras Red breeds. Marwari was most distant from rest ofthe 3 breeds by genetic distance estimate. This study generated the basic RAPD fingerprint data in the above sheep breed.

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Author Biographies

  • K GANESH KUMAR
    Assistant Professor, LRS, Kattuppakkam,
    TNVASU, Chennai.

  • PUSHPENDRA KUMAR
    Senior Scientist, Animal Genetic Division.
  • SANJEEV KUMAR
    Senior Scientist, Animal Genetic Division.
  • T K BHATTACHARYA
    Scnior Scientist, CARI, Izatnagar.
  • B BHUSHAN
    Director, NBAGR, Karnal.
  • S P S AHLAWAT

    Senior Scientist, Animal Genetic Division.

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Issue

Vol. 74 No. 8 (2004)

Section

Articles

License

The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.

How to Cite

KUMAR, K. G., KUMAR, P., KUMAR, S., BHATTACHARYA, T. K., BHUSHAN, B., & AHLAWAT, S. P. S. (2014). Randomly amplified polymorphic DNA (RAPD) fingerprinting of Indian sheep breeds*. The Indian Journal of Animal Sciences, 74(8). https://epubs.icar.org.in/index.php/IJAnS/article/view/39143