Effect of additives on enzyme leakage during cryopreservation of bull semen*


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Authors

  • SUSHANT SRIVASTAVA
  • SATISH KUMAR

Keywords:

Animal reproduction, Cryopreservation, Enzyme, Semen

Abstract

A study was conducted to evaluate the effect of ascorbic acid, caffeine and chloroquine diphosphate on the enzymes
(GOT, GPT. ACP and AKP) leakage from spermatozoa of crossbred (Holstein Friesian x Haryana) and Holstein Friesian
bull during freezing and thawing process. The mean activity of GOT. GPTand ACP in neat semen did not differ significantly
between breeds and between bulls within the breed. However, activity of AKP differed signifiqantly (P<0.05) between crossbred bulis. lncorporation of additives resulted in low enzymes leakage during cryopreservation. Leakage, of enzymes was minimum in presence of ascorbic acid followed by chlbroquine as compared to caffeine and control groups.

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Author Biographies

  • SUSHANT SRIVASTAVA
    Senior Research Fellow, Project directorate on
    Cattle, Meerut Cantt, Meerut.
  • SATISH KUMAR
    Senior Scientist, Animal Reproduction Division.

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Issue

Vol. 74 No. 9 (2004)

Section

Articles

License

The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.

How to Cite

SRIVASTAVA, S., & KUMAR, S. (2014). Effect of additives on enzyme leakage during cryopreservation of bull semen*. The Indian Journal of Animal Sciences, 74(9). https://epubs.icar.org.in/index.php/IJAnS/article/view/39166