Reduced glutathione, glucose-6-phosphate dehydrogenase and hemoglobin in Indian buffaloes

Abstract

The reduced glutathione, G-6-PD enzyme activity and hemoglobin level were estimated in 6 different breeds of buffaloes. The overall reduced glutathione level in buffaloes was 19.76±0.12 mg/100 ml blood, with the highest in Murrah (22.79±0.35) followed by Badhawari (21.64±0.48), Nili-Ravi (21.49±0.72), Surti (19.76±0.53), Jaffarabadi (19.35±0.46) and lowest in non-descript breeds as 18.86±0.14. The effect of breed was highly significant on reduced glutathione level. Higher level of reduced glutathione was in young animals as compared to calves and adults. Three G- 6-PD phenotypes determined by screening test on the basis of blood enzyme activity level were normal, heterozygote and deficient types. The G-6-PD activity was highest in the normal phenotype and lowest in deficient phenotype. Whereas the heterozygote phenotype was intermediate. The effect of breeds, age groups, health status and G-6-PD phenotypes were significant on hemoglobin and reduced glutathione while the effect of breeds, age groups and sex was nonsignificant on G-6-PD activity. Healthy animals showed higher levels of G-6-PD activity, reduced glutathione level and haemoglobin concentration. Significant correlations were found between reduced glutathione level with hemoglobin and G-6-PD activity in different breeds, age groups, health status and sex.