Haemagglutinating activity of canine parvovirus


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Authors

  • PRAVEEN KUMAR
  • S K GARG
  • S K BANDYOPADHYAY
  • RASHMI SINGH
  • SAMEER SHRIVASTAVA

Keywords:

Canine, CPV, Haemagglutinating activity, CRFK, Parvovirus

Abstract

Haemagglutination is an important characteristic of non-defective parvovirus. The HA test is simple, convenient and comparatively more accurate. The present study was aimed to standardize the HA test using erythrocytes of different species, and to analyze the effect of different incubation temperatures, buffers and pH on the HA reaction. The porcine erythrocytes when used at an incubation temperature of 4oC gave best results. Highest HA titer was obtained using PBS, PBSS and PBS with 0.1 % BSA as diluent in the pH range of 4-6. Also its specificity was ascertained by haemagglutination inhibition test and a HI titer of 3120 was obtained. Using the standardized reagents, HA test was used to detect CPV in the fecal samples from clinical cases and experimentally infected pup.

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Author Biographies

  • PRAVEEN KUMAR
    In charge, Disease Investigation Laboratory, NDRI, Karnal 132 001
  • S K GARG
    Dean, PGS,
  • S K BANDYOPADHYAY
    Ph.D. Scholar, National Biotechnology Centre IVRI Izatnagar 243 122
  • RASHMI SINGH
    Head, Division of Virology, IVRI, Mukteswar 263 138
  • SAMEER SHRIVASTAVA
    Junior Research Officer, Department of Microbiology, College of Veterinary Science.

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Issue

Vol. 73 No. 2 (2003)

Section

Articles

License

The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.

How to Cite

KUMAR, P., GARG, S. K., BANDYOPADHYAY, S. K., SINGH, R., & SHRIVASTAVA, S. (2014). Haemagglutinating activity of canine parvovirus. The Indian Journal of Animal Sciences, 73(2). https://epubs.icar.org.in/index.php/IJAnS/article/view/42749