Comparative efficacy of different diluents on liquid preservation of boar semen

Abstract

The study was carried out to find the effect ofdifferent extenders on preservation of boar semen in liquid condition. Ejaculates (60-15 each from 4 Hampshire boars) were collected twice weekly using dummy sow. The ejaculates having more than 80% initial progressive motility were diluted in 4 different extenders, viz. Belts Ville thawing solution (BTS) Modena, Zomobsa and Guelph/Kiev. The diluted semen was preserved at 18°C up to 96 h. The average sperm motility was 48.08, 43.83,40.56 and 33.41 %, respectively, in BTS, Modena, Zomobsa and Guelph after 96 h of preservation. While live percentage, acrosomal integrity and hypo-osmotic swollen spermatozoa were 53.45, 55.47 and 25.60%, respectively, in BTS; 49.46, 50.06 and 20.56% in Modena; 43.21,44.63 and 19.85% in Zornobsa; and 37.40,40.52 and 19.53% in Guelph extender after 96 h of preservation at 18°C. There was no significant difference observed between BTS and Modena as well as between Zornobsa and Guelph extenders. The results concluded that BTS and Modena are better extenders than Zornobsa and Guelph in terms of motility, viability, acrosomal integrity and hypo-osmotic swollen spermatozoa for liquid preservation of boar semen.