Molecular typing of Indian isolates of Pasteurella multocida serogroup-A from different animal species

Abstract

Fifty strains ofPasteurella multocida serogroup-A isolated from various animal species in India, were characterized by using REA, AFLP and RAPD-PCR techniques. REA using HinjI, HindUI and Nco/provided appreciable resolution ofbands, however, HinjI appeared to be the most suitable enzyme that produced a clear cleavage pattern resulting in 39 distinct profiles. Single enzyme AFLP analysis using non-selective HindU! primer resulted in evenly distributed band patterns for amplified fragments ranging from 300 to 3000 bp. Thirty-one isolates ofserotype A:I were grouped into 22 AFLP patterns. Avian isolates showed maximum diversity with 12 patterns, followed by cattle and buffaloes with 6 and sheep with 2 distinct profiles. The RAPD primers sllccessfullytyped all the 50 isolates with varying number ofobserved bands. Maximum differentiating RAPD patterns were found among isolates ofA: 1 serotype, followed by A:3 serotype. Species-wise, avian isolates were more diversified, followed by isolates from larger ruminants and subsequently those from small ruminants. The current study demonstrates the value of REA, AFLP and RAPD analyses in the molecular typing ofP. multocida serogroup-A isolates from multiple animal species.