Random amplified polymorphic DNA (RAPD) analysis ofMycobacterium bovis strain in India


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Authors

  • J P N Singh
  • Rishendra Verma
  • P Chaudhuri

Keywords:

India, Mycobacterium bovis, Random primer, RAPD, Typing

Abstract

The usefulness of random amplification of polymorphic DNA (RAPD) analysis for typing Indian strains of Mycobacterium bovis was mvestigated. M bovis ANs' M. bovis BCG and 20 field strains of M. bovis were subjected to RAPD-PCR Using 7 random decamer primers. All 7 primers were differentiative and produced specific RAPD profiles. The polymorphic amplication served as RAPD markers for M. bovis. The dendrograms, obtained by different primers, showed the discrimatory ablity of the rinmers. RAPD analysis provided a rapid and easy means of identifying polymorphIsm M. bovis and It was a valuable alternative epidemiological tool. In addition, the results ofthe present study showed heterogeneIty mthe M bovis strains in the population studied.

 

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Issue

Vol. 76 No. 11 (2006)

Section

Articles

License

The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.

How to Cite

Singh, J. P. N., Verma, R., & Chaudhuri, P. (2011). Random amplified polymorphic DNA (RAPD) analysis ofMycobacterium bovis strain in India. The Indian Journal of Animal Sciences, 76(11). https://epubs.icar.org.in/index.php/IJAnS/article/view/4455