Comparative analysis of outer membrane protein profile of Pasteurella multocida serotype B:2 grown under iron regulated conditions

Abstract

The present study was undertaken to study the protein profile ofPasteurella multocida grown under iron regulated condition. SDS-PAGE analysis of aMP's of P multocida serotype B:2 Brown on BHI agar and iron replete conditions revealed more than 12 protein bands ranging from 26 to 100 kDa molecular weight and in iron deplete conditions additiona12 protein bands with molecular weight of 101 kDa and 104 kDa were observed. On the basis of stain intensity 32 kDa protein appeared to be major protein band. Immunoblotting studies carried out by homologous antisera revealed that 32 kDa protein band was immunodominant. In case of nitrocellulose membrane probed with homologous convalescent sera raised in iron deplete conditions, additional protein bands of 101 kDa and 104 kDa molecular weight were immunodominant.