Dectation and characterziation of Mycobacterium avium subspecies paratuberculosis from Vaginal secretions post-parturient farm goats using culture, IS 900 and ELISA kit

Abstract

Vaginal secretions and sera fronl 29 post-parturient farm goats were secreened by culture, IS900 peR and indigenous ELISA kit to know the presence of MAP in reproductive tract after parturition. Decontaminated pellets from vaginal secretions and ,MAP cultures were processed for DNA isolation and screened by 18900 peR. Positive DNA samples on amplification yielded specific 229 bp band from pellets of vaginal secretions. Of 10 DNAs obtained from 29 pellets, 4 were amplified. Soluble protoplasmic (PPA) antigen from MAP 'Bison type' strain cultured from tenninal case of JD in goat was used in ELISA kit. Vaginal secretions (VS) 37.9% each were positive in culture and ELISA from 29 goats of farm herds. Of the 3 tests, culture was the most sensitive. There was poor correlation (37.50/0) between culture of VS and strong positives in ELISA kit. However, correlation was good between positives, low positives and suspected goats in SIP ratio with culture of VS. This study was maiden attempt and reports high presence and recovery of MAP in vaginal secretions of post parturient fann goats in India by culture and ELISA kit. Positive IS900 peR in DNA pellets confirmed presence of MAP. Vaginal secretion was potential source of contamination to new born kids and animals handlers and was a good clinical material to screeen post parturient female goats. It also indicated potential contamination of fetus during pregnancy in JD infected goats.