A rapid method for peR amplification of specific genes of Mycobacterium avium sub sp. paratuberculosis


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Authors

  • K VIJAYARANI
  • K KUMAMAN

Keywords:

Mycobacterium avium sub sp. paratuberculosis, Johne's disease, Paratuberculosis, Polymerase chain reaction

Abstract

The present study describes a simple and rapid method for amplifying various genes of Mycobacterium aviLl1n subsp. paratuberculosis (MAP) by polymerase chain reaction. Culture supernatants of MAP, when used as template resulted in the amplification of IS 900, ISMav2, ISMap02, and superoxide dismutase genes of MAP. This Inethod circumvents the specific isolation of genomic DNA frOln concentrated MAP organisms for ainplifying MAP genes and saves valuable reagents and time.

 

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Issue

Vol. 78 No. 10 (2008)

Section

Articles

License

The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.

How to Cite

VIJAYARANI, K., & KUMAMAN, K. (2011). A rapid method for peR amplification of specific genes of Mycobacterium avium sub sp. paratuberculosis. The Indian Journal of Animal Sciences, 78(10). https://epubs.icar.org.in/index.php/IJAnS/article/view/4873