In-vitro fertilizer and cleavage rate of in vitro matured prepubertal and pubertal goat oocytes matured in maturation media with different supplementation

Abstract

The experiment was designed to evaluate effect of different media additives in IVP. Cleavage rates of goat oocytes matured in-vitro with basic media TCM-199 supplemented with either concentration of EGS, or 20% NCS, or 100/0 NSC plus horn1one (FSH @ 211g/ml + LH @ 100 IU ml+E2 @ Illg/ml) or 20% CFF. After 27 h the matured oocytes were picked up and denuded by treating them with 0.1% hyaluronidase and by repeated pipetting through a fine boar pipette. The denuded oocytes were transferred in fert-TALP medium (fertilization medium). The capacitated sperm having concentration of lxl06 live sperm/rol were used for in-vitro fertilization. Oocytes were co-incubated with spermatozoa for 18 h at 38.5±1°C in an atmosphere of 5% CO2 in a humidified air. After 48 h of insemination the cleavage rates of 43.30~ 41.08, 42.07 and 28.68, 41.95, 44.06, 40.37% were obtained for prepubertal and pubertal goat oocytes, respectively, in different treatment groups of the maturation medium when the fertilization medium was kept the same.