Diagnosis of bovine herpesvirus-l (BHV-l) infection in aborting cattle and buffaloes by virus isolation and polymerase chain reaction (PCR)


199 / 53

Authors

  • J S DRAMI
  • P N DWIVEDI
  • RAMNEEK .
  • DIPAK DEKA
  • N K MAITI
  • M S OBEROI

Keywords:

Abortion, Bovine herpes virus 1, Buffaloes, Cattle, Enzyn1e linked in1munosorbent assay,

Abstract

Sera samples (50) from cattle and buffaloes with the history of abortion from 2 dairy farms of Punjab were screened for BHV-1 antibody by using avidin-biotin ELISA (AB-ELISA) that revealed an overall prevalence rate of 72%. The seroprevalence of BHV-1 in cattle and buffaloes was 85.70 and 54.50%, respectively. Subsequently, double antibody sandwich ELISA (DAS-ELISA), virus isolation and peR were carned out to detect BHV-1 in aborted placental tissues. A marked difference was observed between the proportion of peR positive and DAS-ELISA and virus isolation positive sanlples, suggesting peR to be more sensitive method for detection ofBHV-1 in aborted placental tissue.

 

Downloads

Download data is not yet available.

Downloads

Issue

Vol. 78 No. 11 (2008)

Section

Short-Communication

License

The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.

How to Cite

DRAMI, J. S., DWIVEDI, P. N., ., R., DEKA, D., MAITI, N. K., & OBEROI, M. S. (2011). Diagnosis of bovine herpesvirus-l (BHV-l) infection in aborting cattle and buffaloes by virus isolation and polymerase chain reaction (PCR). The Indian Journal of Animal Sciences, 78(11). https://epubs.icar.org.in/index.php/IJAnS/article/view/4959