Evaluation of an ELISA using recombinant outer membrane protein 31 for diagnosis of ovine brucellosis


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Authors

  • V K GUPTA
  • JYOTI VOHRA
  • RANJEETA KUMARI
  • V S VIHAN

Keywords:

Brucellosis, Recombinant outer membrane protein 31

Abstract

An enzyme-linked immunosorbent assay (ELISA) was developed for the serological diagnosis of ovine brucellosis with purified recombinant outer membrane protein 31 (rOmp31) of Brucella melitensis 16M produced in Escherichia coli. The rOmp 31 ELISA performed with serum samples (n=1152) obtained from organized sheep farm from Northern India showed a seroprevalence of 11.45%. Results were compared with the traditional Rose Bengal Plate test. Thus, these results confirmed the importance of omp31 as a suitable antigen for the serological diagnosis of ovine brucellosis.

 

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Issue

Vol. 79 No. 1 (2009)

Section

Short-Communication

License

The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.

How to Cite

GUPTA, V. K., VOHRA, J., KUMARI, R., & VIHAN, V. S. (2011). Evaluation of an ELISA using recombinant outer membrane protein 31 for diagnosis of ovine brucellosis. The Indian Journal of Animal Sciences, 79(1). https://epubs.icar.org.in/index.php/IJAnS/article/view/5144