Potential of fungal exopolysaccharide as novel source for prebiotic supplement to broiler chicken diet
461 / 133
Authors
-
W PRATHUMPAI
Researcher, 113 Thailand Science Park, Phahonyothin Road, Klong Nueng, Klong Luang, Pathum Thani 12120 Thailand
-
P RACHTAWEE
Research Assistant, Bioresources Technology Unit, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency
-
S KHAJEERAM
113 Thailand Science Park, Phahonyothin Road, Klong Nueng, Klong Luang, Pathum Thani 12120 Thailand
Keywords:
Beta-glucan, Denaturing gradient gel-electrophoresis, Exopolysaccharide, Ophiocordyceps dipterigena, PrebioticAbstract
The potential of using a fungal exopolysaccharide produced by Ophiocordyceps dipterigena BCC 2073, as a prebiotic in broiler chicken diet by supplementing the diet at different concentrations (0-120 g/25 kg feed) was evaluated over a period of 42 days. Intestinal microbial populations in faeces were identified. The effects of a combination of pre- and pro-biotic supplements were also studied by combining exopolysaccharide with Lactobacillus acidophilus BCC 13938. Total Lactobacilli sp. counts in faecal samples of chickens fed diet containing both exopolysaccharide and L. acidophilus BCC 13938 were 50-folds higher than the non prebiotic but probiotic supplemented groups. Total body weight, body weight increase, feed intake, feed conversion efficiency and growth rate of chickens on a diet supplemented with 150 g of L. acidophilus BCC 13938 plus 1.5 kg of water or 30 g of O. dipterigena BCC 2073 exopolysaccharide plus 150 g of L. acidophilus BCC 13938 plus 0.75 kg of water were higher than those fed other combinations of supplements. The microbial community of faecal samples from chickens supplementation with exopolysaccharide had reduced numbers of E. coli, Staphylococcus sp., Streptococcus sp., Enterococcus sp. and increased numbers of Lactobacilli and Bacilli. The numbers of faecal pathogenic bacteria inversely correlated with total body weight, body weight increase, feed intake, feed conversion efficiency and growth rate. This is the first report on the benefit of fungal exopolysaccharide as a novel prebiotic dietary supplement to chicken.
Downloads
References
Abdel-Raheem S, Abd-Allah S M S, Hassanein M A. 2012 The effects of prebiotic, probiotic and synbiotic supplementation on intestinal microbial ecology and histomorphology of broiler chickens. International Journal for Agro Veterinary and Medical Sciences 6: 277–89. DOI: https://doi.org/10.5455/ijavms.156
Bruzzese E, Volpicelli M, Squeglia V, Bruzzese D, Salvini F, Bisceglia M, Lionetti P, Cinquetti M, Iacono G, Amarri S and Guarino A. 2009. A formula containing galacto- and fructooligosaccharides prevents intestinal and extra-intestinal infections: an observational study. Clinical Nutrition 28: 156– 61. DOI: https://doi.org/10.1016/j.clnu.2009.01.008
Coviello T, Palleschi A, Grassi M, Matricardi P, Bocchinfuso G, Alhaique F. 2005. Scleroglucan: a versatile polysaccharide for modified drug delivery. Molecules 10: 6–33. DOI: https://doi.org/10.3390/10010006
El-Banna H A, El-Zorba H Y, Attia T A, Elatif A A. 2010. Effect of probiotic, prebiotic and synbiotic on broiler performance. World Applied Science Journal 11: 388–93.
Falony G, Lazidou K, Verschaeren A, Weckx S, Maes D and De Vuyst L. 2009. In vitro kinetic analysis of fermentation of prebiotic inulin-type fructans by Bifidobacterium species reveals four different phenotypes. Applied and Environmental Microbiology 75: 454–61. DOI: https://doi.org/10.1128/AEM.01488-08
Funami T, Yada H and Nakao Y. 1998. Curdlan properties for application in fat mimetics for meat products. Journal of Food Science 63: 283–87. DOI: https://doi.org/10.1111/j.1365-2621.1998.tb15727.x
Geraylou Z, Souffreau C, Rurangwa E, D’Hondt S, Callewaert L, Courtin C M, Delcour J A, Buyse J and Ollevier F. 2012. Effects of arabinoxylan-oligosaccharides (AXOS) on juvenile Siberian sturgeon (Acipenser baerii) performance, immune responses and gastrointestinal microbial community. Fish Shellfish Immunology 33: 718–24. DOI: https://doi.org/10.1016/j.fsi.2012.06.010
Hernot D C, Boileau T W, Bauer L L, Middelbos I S, Murphy M R, Swanson K S and Fahey G C Jr. 2009. In vitro fermentation profiles, gas production rates, and microbiota modulation as affected by certain fructans, galactooligosaccharides, and polydextrose. Journal of Agricultural and Food Chemistry 57: 1354–61. DOI: https://doi.org/10.1021/jf802484j
Iyer A, Mody K and Jha B. 2006. Emulsifying properties of a marine bacterial exopolysaccharide. Enzyme and Microbial Technology 38: 220–22. DOI: https://doi.org/10.1016/j.enzmictec.2005.06.007
Jung S J, Houde R, Baurhoo B, Zhao X and Lee B H. 2008. Effects of galacto-oligosaccharides and a Bifidobacteria lactis- based probiotic strain on the growth performance and fecal microflora of broiler chickens. Poultry Science 87: 1694– 99. DOI: https://doi.org/10.3382/ps.2007-00489
Kocharin K, Rachathevee P, Sanglier J J and Prathumpai W. 2010. Exobiopolymer production of Ophiocordyceps dipterigena BCC 2073: optimization, scaling-up, and characterization. BMC Biotechnology 10: 51. DOI: https://doi.org/10.1186/1472-6750-10-51
Li P, Burr G S, Gatlin D M, Hume M E, Patnaik S, Castille F L and Lawrence A L. 2007. Dietary supplementation of shortchain fructooligosaccharides influences gastrointestinal microbiota composition and immunity characteristics of Pacific white shrimp, Litopenaeus vannamei, cultured in a recirculating system. Journal of Nutrition 137: 2763–68. DOI: https://doi.org/10.1093/jn/137.12.2763
Lomax A R and Calder PC. 2009. Prebiotics, immune function, infection and inflammation: a review of the evidence. British Journal of Nutrition 101: 633–58. DOI: https://doi.org/10.1017/S0007114508055608
Madla S, Methacanon P, Prasitsil M and Kirtikara K. 2005. Characterization of biocompatible fungi-derived polymers that induce IL-8 production. Carbohydrate Polymer 59: 275–280. DOI: https://doi.org/10.1016/j.carbpol.2004.07.002
Methacanon P, Madla S, Kirtikara K and Prasitsil M. 2005. Structural elucidation of bioactive fungi-derived polymers. Carbohydrate Polymer 60: 199–203. DOI: https://doi.org/10.1016/j.carbpol.2004.12.006
Mookiah S, Sieo C C Ramasamy K, Abdullah N, Ho Y W. 2014. Effects of dietary prebiotics, probiotic and synbiotics on performance, caecal bacterial populations and caecal fermentation concentrations of broiler chickens. Journal of the Science of Food and Agriculture 94: 341–48. DOI: https://doi.org/10.1002/jsfa.6365
Prathumpai W, Rachathewee P, Khajeeram S, Sanglier J J, Tanjak, P, Methacanon P. 2013. Optimization, characterization and in Vitro evaluation of entomopathogenic fungal exopolysaccharides as prebiotic. Advances in Biochemistry 1: 13–21. DOI: https://doi.org/10.11648/j.ab.20130102.12
Rehman H, Hellweg P, Taras D and Zentek J. 2008. Effects of dietary inulin on the intestinal short chain fatty acids and microbial ecology in broiler chickens as revealed by denaturing gradient gel electrophoresis. Poultry Science 87: 783–89. DOI: https://doi.org/10.3382/ps.2007-00271
Salazar N, Ruas-Madiedo P, Kolida S, Collins M, Rastall R, Gibson G and de Los Reyes-Gavilán C G. 2009. Exopolysaccharides produced by Bifidobacterium longum IPLA E44 and Bifidobacterium animalis subsp. lactis IPLA R1 modify the composition and metabolic activity of human faecal microbiota in pH-controlled batch cultures. International Journal of Food Microbiology 135: 260–67. DOI: https://doi.org/10.1016/j.ijfoodmicro.2009.08.017
Saulnier D M, Spinle, J K, Gibson G R and Versalovic J. 2009. Mechanisms of probiosis and prebiosis: considerations for enhanced functional foods. Current Opinion in Chemical Biology 20: 135–41. DOI: https://doi.org/10.1016/j.copbio.2009.01.002
Shen J, Zhang B, Wei H, Che C, Ding D, Hua X, Bucheli P, Wang L, Li Y, Pang X and Zhao L. 2010. Assessment of the modulating effects of fructo-oligosaccharides on fecal microbiota using human flora-associated piglets. Archives of Microbiology 192: 959–68. DOI: https://doi.org/10.1007/s00203-010-0628-y
Shingel K I. 2004. Current knowledge on biosynthesis, biological activity, and chemical modification of the exopolysaccharide, pullulan. Carbohydrate Research 339: 447–60. DOI: https://doi.org/10.1016/j.carres.2003.10.034
Sutherland I W. 1990. Biotechnology of Microbial Exopolysaccharides. Cambridge University press, Cambridge, UK. DOI: https://doi.org/10.1017/CBO9780511525384
Vinarta S C, Molina O E, Figueroa L I C and Farina J I. 2006. A further insight into the practical applications of exopolysaccharides from Sclerotium rolfsii. Food Hydrocolloids 20: 619–69. DOI: https://doi.org/10.1016/j.foodhyd.2005.05.006
Yoo S D and Harcum S W. 1999. Xanthan gum production from waste sugar beet pulp. Bioresource Technology 70: 105–09. DOI: https://doi.org/10.1016/S0960-8524(99)00013-9
Downloads
Submitted
Published
Issue
Section
License
Copyright (c) 2015 The Indian Journal of Animal Sciences
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.
How to Cite
