Clinical and haematological findings in experimental reproduction of CIA and co'ofirmation by electron microscopy

Abstract

In the present study an outbreak suspected of ClA was investigated in one of the organized poultry farms (flock size of23,000, aging 13 weeks). Convalescent serum samples from the affected flock were tested by ELISA and confirmed as CIA. Bone-marrow, thymus, bursa, spleen and liver suspensions were experimentally infected in 3 different groups of 70 birds each. Group 1 (70) chicks were inoculated with tissue suspension and p-methasone. Group 2 chicks (70) were inoculated with tissue suspension alone. Group 3 (70) chicks were treated as control by inoculating with distilled water. The affected chicks showed the signs of weakness, emasciation, weight loss, paleness of combs and wattles. The infection was further evidenced by aplasia of bone-marrow with yellowish white gelatinous consistency, atrophy of thymus and bursa, paleness ofUver and spleen were observed. Further bone-marrow, plasma and liver samples collected from experimentally infected birds between 12 and 15 days PI were subjected to electron microscopy. Many viral particles of 20.2 to 26.2 nm were detected in bone-marrow, plasma and liver in order of decreasing concentration.