Isolation and characterization ofserum immunoglobulins of Cyprinus carpio


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Authors

  • NEERAJ SOOD
  • GAURAV RATHORE
  • T RAJA SWAMINATHAN
  • REHANA ABIDI
  • B N MISHRA
  • W S LAKRA

Keywords:

Chromatography, Cyprinus carpio, Immunoglobulin, SDS..PAGE

Abstract

Common carp (Cyprinus carpio) immunoglobulin (Ig) was purified from serum by affinity chromatography using bovine serum albumin as capture ligand. The purified Ig had a molecular weight (MW) of820 kDa as determined by gel filtration chromatography. The MW of heavy and light chain of common carp Ig was 73.7 and 25.3 kDa, respectively, in SDS-PAGE. In no~-reducing SDS..PAGE, 3 bands of different MW were observed, which were presumed to be of different forms of Ig.

 

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Issue

Vol. 77 No. 7 (2007)

Section

Short-Communication

License

The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.

How to Cite

SOOD, N., RATHORE, G., SWAMINATHAN, T. R., ABIDI, R., MISHRA, B. N., & LAKRA, W. S. (2011). Isolation and characterization ofserum immunoglobulins of Cyprinus carpio. The Indian Journal of Animal Sciences, 77(7). https://epubs.icar.org.in/index.php/IJAnS/article/view/5529