Effect of solid state fermentation on nutrient content and ileal amino acids digestibility of palm kernel cake in broiler chickens

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  • MOHAMED IDRIS ALSHELMANI Universiti Putra Malaysia, Serdang, Selangor 43400 Malaysia
  • TECK CHWEN LOH Universiti Putra Malaysia, Serdang, Selangor 43400 Malaysia
  • HOOI LING FOO Universiti Putra Malaysia, Serdang, Selangor 43400 Malaysia
  • AWIS QURNI SAZILI Universiti Putra Malaysia, Serdang, Selangor 43400 Malaysia
  • WEI HONG LAU Universiti Putra Malaysia, Serdang, Selangor 43400 Malaysia



Amino acids, Broiler, Cellulolytic bacteria, Digestibility, Fermented palm kernel cake


Digestibility trial was conducted to determine the apparent ileal digestibility (AID) of crude protein (CP) and amino acids (AA) in untreated palm kernel cake (PKC) and fermented palm kernel cake (FPKC) on finisher broiler. Paenibacillus polymyxa ATCC 842 and P. curdlanolyticus DSMZ 10248 were used to produce FPKCa and FPKCb, respectively through solid state fermentation (SSF). Broiler male chickens were fed with diets containing 15% PKC from day one until 41 days of age. Birds (36) were selected with uniform body weight, and randomly distributed into 3 groups with 6 replicates in each treatment and 2 birds per replicate. The chickens were deprived from food overnight with free access to drinking water. The birds were fed PKC, FPKCa and FPKCb with indigestible marker. All the chickens were allowed free access to the test ingredients and drinking water for 4 days. The birds were slaughtered; ileal digesta were individually collected, pooled within each replicate in plastic cups; and immediately kept at -20°C for chemical analysis. The findings showed that the process of SSF by cellulolytic bacteria increased the levels of CP from 16.43% in the PKC to 16.68% and 16.80% in FPKCb and FPKCa, respectively. The AID of CP was increased in FPKC compared to the PKC. Additionally, there was an increase in the digestibilities of AA in FPKC compared to untreated PKC. The process of SSF decreased the fibres in FPKC, and there was improvement in the nutrient value of FPKC by cellulolytic bacterial cultures in terms of nutrient content and digestibility.


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Alvarez-Cervantes J, Hern´andez-Dom´ýnguez E M, Arana- Cuenca A, D´ýaz-God´ýnez G and Mercado-Flores Y. 2013. Purification and characterization of xylanase SRXL1 from sporisorium reilianum grown in submerged and solid-state fermentation. BioResources 8: 5309–18. DOI: https://doi.org/10.15376/biores.8.4.5309-5318

Adebiyi A O and Olukosi O A. 2015. Apparent and standardised ileal amino acid digestibility of wheat distillers dried grains with solubles with or without exogenous protease in broilers and turkeys. British Poultry Science 56: 239–46. DOI: https://doi.org/10.1080/00071668.2015.1011606

Ahmed A, Zulkifli I, Farjam A S, Abdullah N, Liang J B and Awad E A. 2014. Effect of solid state fermentation on nutrient content and ileal amino acids digestibility of canola meal in broiler chickens. Italian Journal of Animal Science 13: 410– 14. DOI: https://doi.org/10.4081/ijas.2014.3293

Alimon A R. 2004. The nutritive value of palm kernel cake for animal feed. Palm Oil Development 40: 12–14.

Alshelmani M I, Loh T C, Foo H L, Lau W H and Sazili A Q. 2013. Characterization of cellulolytic bacterial cultures grown in different substrates. The Scientific World Journal 2013: 6. DOI: https://doi.org/10.1155/2013/689235

Alshelmani M I, Loh T C, Foo H L, Lau W H and Sazili A Q. 2014. Biodegradation of palm kernel cake by cellulolytic and hemicellulolytic bacterial cultures through solid state fermentation. The Scientific World Journal 2014: 8. DOI: https://doi.org/10.1155/2014/729852

Alshelmani M I, Loh T C, Foo H L, Sazili A Q and Lau W H. 2016a. Effect of feeding different levels of palm kernel cake fermented by Paenibacillus polymyxa ATCC 842 on nutrient digestibility, intestinal morphology, and gut microflora in broiler chickens. Animal Feed Science and Technology 216: 216–24. DOI: https://doi.org/10.1016/j.anifeedsci.2016.03.019

Alshelmani M I, Loh T C, Foo H L, Sazili A Q and Lau W H. 2016b. Effect of feeding different levels of palm kernel cake fermented by Paenibacillus polymyxa ATCC 842 on broiler growth performance, blood biochemistry, carcass characteristics, and meat quality. Animal Production Science 57: 839-48. DOI: https://doi.org/10.1071/AN15359

Alvarez V M, Von Der Weid I, Seldin L and Santos A L S. 2006. Influence of growth conditions on the production of extracellular proteolytic enzymes in Paenibacillus peoriae NRRL BD-62 and Paenibacillus polymyxa SCE2. Letters in Applied Microbiology 43: 625–30. DOI: https://doi.org/10.1111/j.1472-765X.2006.02015.x

Annison G and Choct M. 1991. Anti-nutritive activities of cereal non-starch polysaccharides in broiler diets and strategies minimizing their effects. World’s Poultry Science Journal 47: 232–42. DOI: https://doi.org/10.1079/WPS19910019

AOAC. 1995. Official Methods of Analysis (16th ed.). Arlington, VA. Association of Official Analytical Chemists.

Aya V, Ayanwale B, Ijaiya A and Aremu A. 2013. Performance and nutrient digestibility in broiler chicks as influenced by multienzyme addition to starter diets containing palm kernel meal. Biotechnology in Animal Husbandry 29: 93–104. DOI: https://doi.org/10.2298/BAH1301093A

Chen W L, Liang J B, Jahromi M F, Ho Y W and Abdullah N. 2013. Optimization of multi-enzyme production by fungi isolated from palm kernel expeller using response surface methodology. BioResources 8: 3844–57. DOI: https://doi.org/10.15376/biores.8.3.3844-3857

Dégen L, Halas V and Babinszky L. 2007. Effect of dietary fibre on protein and fat digestibility and its consequences on diet formulation for growing and fattening pigs: A review. Acta Agriculturae Scandinavica, Section A — Animal Science 57: 1–9. DOI: https://doi.org/10.1080/09064700701372038

Fadil M, Alimon A R, Meng G Y, Ebrahimi M and Farjam A S. 2014. Palm kernel cake as a potential ingredient in Muscovy ducks diet. Italian Journal of Animal Science 13: 112–15. DOI: https://doi.org/10.4081/ijas.2014.3035

Gao Y L, Wang C S, Zhu Q H and Qian G Y. 2013. Optimization of solid-state fermentation with Lactobacillus brevis and Aspergillus oryzae for trypsin inhibitor degradation in soybean meal. Journal of Integrative Agriculture 12: 869–76. DOI: https://doi.org/10.1016/S2095-3119(13)60305-6

Goering H K and Van Soest P J. 1970. Forage fibre analysis (apparatus, reagents, prosedures and some applications). USDA. Agricultural Handbook. No 379

Graminha E B N, Gonçalves A Z L, Pirota R D P B, Balsalobre M A A, Da Silva R and Gomes E. 2008. Enzyme production by solid-state fermentation: Application to animal nutrition. Animal Feed Science and Technology 144: 1–22. DOI: https://doi.org/10.1016/j.anifeedsci.2007.09.029

Iluyemi F B, Hanafi M M, Radziah O and Kamarudin M S. 2006. Fungal solid state culture of palm kernel cake. Bioresource Technology 97: 477–82. DOI: https://doi.org/10.1016/j.biortech.2005.03.005

Iyayi E A and Davies B I. 2005. Effect of enzyme supplementation of palm kernel meal and brewer’s dried grain on the performance of broilers. International Journal of Poultry Science 4: 76–80. DOI: https://doi.org/10.3923/ijps.2005.76.80

Khin H S. 2004. ‘Evaluation of solid state fermentation by Aspergillus niger to improve the nutritive value of palm kernel cake for broilers.’ Ph. D. Thesis. Universiti Putra Malaysia.

Kocher A, Choct M, Porter M D and Broz J. 2002. Effects of feed enzymes on nutritive value of soyabean meal fed to broilers. British Poultry Science 43: 54–63. DOI: https://doi.org/10.1080/00071660120109890

Lateef A, Oloke J K, Gueguim Kana E B, Oyeniyi S O, Onifade O R, Oyeleye A O, Oladosu O C and Oyelami A O. 2008. Improving the quality of agro-wastes by solid-state fermentation: enhanced antioxidant activities and nutritional qualities. World Journal of Microbiology and Biotechnology 24: 2369–74. DOI: https://doi.org/10.1007/s11274-008-9749-8

Lawal T E, Iyayi E A, Adeniyi B A and Adaramoye O A. 2010. Biodegradation of palm kernel cake with multienzyme complexes from fungi and its feeding value for broiler. International Journal of Poultry Science 9: 695–701. DOI: https://doi.org/10.3923/ijps.2010.695.701

Liu S Y, Selle P H, Court S G and Cowieson A J. 2013. Protease supplementation of sorghum-based broiler diets enhances amino acid digestibility coefficients in four small intestinal sites and accelerates their rates of digestion. Animal Feed Science and Technology 183: 175–83. DOI: https://doi.org/10.1016/j.anifeedsci.2013.05.006

Loh T C, Ling H G, Thanh N T, Foo H L, Rajion M A and David S I. 2008. Effects of feeding phytogenic substances and phytase on growth performance and nutrient digestibility of young broilers. Journal of Applied Animal Research 33: 187–92. DOI: https://doi.org/10.1080/09712119.2008.9706924

Marini A M, Daud M J, Noraini S, Jame’ah H and Azahan E A E. 2005. Performance of locally isolated microorganism in degrading palm kernel cake (PKC) fibre and improving the nutritional value of fermented PKC. Journal of Tropical Agriculture and Food Science 33: 311–19.

Masey-O’neill H V, Singh M and Cowieson A J. 2014. Effects of exogenous xylanase on performance, nutrient digestibility, volatile fatty acid production and digestive tract thermal profiles of broilers fed on wheat- or maize-based diet. British Poultry Science 55: 351–59. DOI: https://doi.org/10.1080/00071668.2014.898836

McDonald P, Edwards R A and Greenhalgh J F D. 1995. Animal Nutrition. Longman Singapore Publishers Pvt Ltd.

Mirnawati Y R, Marlida Y and Kompiang P. 2011. Evaluation of palm kernel cake fermented by Aspergillus niger as substituent for soybean meal protein in the diet of broiler. International Jounal of Poultry Science 10: 537–41. DOI: https://doi.org/10.3923/ijps.2011.537.541

Moftah B, Lah T, Nawi A, Kadir M and Aliyu-Paiko M. 2012. Maximizing glucose production from palm kernel cake (PKC) from which residual oil was removed supercritically via solid state fermentation (SSF) method using Trichoderma reesi isolate Pro-AI. The Internet Journal of Microbiology 10(1): 1–7.

Moore S. 1963. On the determination of cystine as cysteic acid. Journal of Biological Chemistry 238: 235–37. DOI: https://doi.org/10.1016/S0021-9258(19)83985-6

Muangkeow N and Chinajariyawong C. 2009. Determination of true amino acid digestibility and metabolizable energy in fermented palm kernel meal with Aspergillus wentii TISTR 3075 for chickens. Walailak Journal of Science and Technology 6: 231–41.

Mustafa M F, Alimon A R, Zahari M W, Idris I and Hair Bejo M. 2004. Nutrient digestibility of palm kernel cake for Muscovy ducks. Asian Australasian Journal of Animal Science 17: 514– 17. DOI: https://doi.org/10.5713/ajas.2004.514

Ng W K and Chong K K. 2002. The nutritive value of palm kernel meal and the effect of enzyme supplementation in practical diets for red hybrid tilapia (Oreochromis sp.). Asian Fisheries Science 15: 167–76. DOI: https://doi.org/10.33997/j.afs.2002.15.2.008

O’Mara F P, Mulligan F J, Cronin E J, Rath M and Caffrey P J. 1999. The nutritive value of palm kernel meal measured in vivo and using rumen fluid and enzymatic techniques. Livestock Production Science 60: 305–16. DOI: https://doi.org/10.1016/S0301-6226(99)00102-5

Pranoto Y, Anggrahini S and Efendi Z. 2013. Effect of natural and Lactobacillus plantarum fermentation on in-vitro protein and starch digestibilities of sorghum flour. Food Bioscience 2: 46–52. DOI: https://doi.org/10.1016/j.fbio.2013.04.001

Ravindran V, Hew L I, Ravindran G and Bryden W L. 2005. Apparent ileal digestibility of amino acids in dietary ingredients for broiler chickens. Animal Science 81: 85–97. DOI: https://doi.org/10.1079/ASC42240085

Ravindran V, Tilman Z V, Morel P C H, Ravindran G and Coles G D. 2007. Influence of α-glucanase supplementation on the metabolisable energy and ileal nutrient digestibility of normal starch and waxy barleys for broiler chickens. Animal Feed Science and Technology 134: 45–55. DOI: https://doi.org/10.1016/j.anifeedsci.2006.04.012

SAS. 2003. Statistical Analytical System. SAS Institute Inc., Cary, NC, USA.

Selle P H, Ravindran V and Partridge G G. 2009. Beneficial effects of xylanase and/or phytase inclusions on ileal amino acid digestibility, energy utilisation, mineral retention and growth performance in wheat-based broiler diets. Animal Feed Science and Technology 153: 303–13. DOI: https://doi.org/10.1016/j.anifeedsci.2009.06.011

Short F, Gorton P, Wiseman J and Boorman K. 1996. Determination of titanium dioxide added as an inert marker in chicken digestibility studies. Animal Feed Science and Technology 59: 215–21. DOI: https://doi.org/10.1016/0377-8401(95)00916-7

Son A R, Hyun Y, Htoo J K and Kim B G. 2014. Amino acid digestibility in copra expellers and palm kernel expellers by growing pigs. Animal Feed Science and Technology 187: 91– 97. DOI: https://doi.org/10.1016/j.anifeedsci.2013.09.015

Sukaryana Y, Atmomarsono U, Yunianto V D and Supriyatna E. 2010. Bioconversions of palm kernel cake and rice bran mixtures by Trichoderma viride toward nutritional contents. International Journal of Science and Engineering 1: 27–32.

Sulabo R C, Ju W S and Stein H H. 2013. Amino acid digestibility and concentration of digestible and metabolizable energy in copra meal, palm kernel expellers, and palm kernel meal fed to growing pigs. Journal of Animal Science 91: 1391–99. DOI: https://doi.org/10.2527/jas.2012-5281

Sundu B and Dingle J. 2002. Use of enzymes to improve the nutritional value of palm kernel meal and copra meal. Proceeding of the Queensland Poultry Science Symposium, Australia.








How to Cite

ALSHELMANI, M. I., LOH, T. C., FOO, H. L., SAZILI, A. Q., & LAU, W. H. (2017). Effect of solid state fermentation on nutrient content and ileal amino acids digestibility of palm kernel cake in broiler chickens. The Indian Journal of Animal Sciences, 87(9), 1135–1140. https://doi.org/10.56093/ijans.v87i9.74331