Isolation and electropherotyping of equine rotaviruses from diarrhoeic foals in India


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Authors

  • Baldev R Gulati National Reasearch Centre on Equines, Sirsa Road, Hisar, Haryana
  • Praveen Malik National Reasearch Centre on Equines, Sirsa Road, Hisar, Haryana
  • Rajender Kumar National Reasearch Centre on Equines, Sirsa Road, Hisar, Haryana

Keywords:

Equine rotavirus, Foal diarrhoea, Isolation, RNA-PAGE

Abstract

Equine rotavirus is one of the major etiological agents associated with diarrhoea in foals. The rotavirus was detected in 4 of 50 (8%) diarrhoeic foals below 2 months of age in a farm near Hisar (Haryana), using RNA-PAGE and group A rotavirus ELISA kit. Rotaviruses were isolated from all the positive samples in MA104 cell lines alter 6-8 blind passages in serum-free MEM medium supplemented with 2 µg/ml af 11 typsin.The RNA profiles of the rotavirus isolates were compared with refrence bovine group A rotaviruses (NCDV, B223, CR129 and CK156). The electrophorectic profile of the equine rotavirus isolates From the outbreak in June 2003 (FRV2 and FRV3) differed from those, of the july outbreak (FRV25 and FRV28) in migration of RNA segment 5. confirming that at least 2 distinct strains of equine rotaviruses are prevalent in the region. This is the first report of isolation and electropherotyping of rotaviruses from diarrhoeic foals in India.

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Submitted

2011-08-01

Published

2005-07-05

Issue

Vol. 75 No. 7 (2005)

Section

Articles

License

The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.

How to Cite

Gulati, B. R., Malik, P., & Kumar, R. (2005). Isolation and electropherotyping of equine rotaviruses from diarrhoeic foals in India. The Indian Journal of Animal Sciences, 75(7). https://epubs.icar.org.in/index.php/IJAnS/article/view/8149