In vitro rumen fermentation and gas production as affected by probiotics addition

Abstract

Promising probiotic cultures or culture combinations taken for it in vitro study, were Saccharomyces cerevisiae-NCDC-47 (Baker's strain) (T1); Enterococcus faecium-NCDC-124 (T2); S, cerevisiae-NCDC-45 (strain-522) and Lactobacillus plantarum-NCDC-25 combination(T3);S cerevisiae-NCDC-45 and E faecium NCDC- 124 combination (T4); S. cerevisiae -NCDC-45, L. acirdophilus-NCDC-15 and Lactococcus lactis-NCDC-94 combination (T5); S. cerevisiae -NCDC-45, L.plantarum-NCDC-25 and E..faecium-NCDC- 124 combination (T6) and S.crerevisiae -NCDC-47, L. plantarum-NCDC-25 and E. faecium -NCDC-124 combination (T7). In control group nO probiotics was added. The in vim bottles were incubated for 24 hr at 39"C*1 degre C. DM degradability was increased due to addition of probiotics in the in vitro bottles. Cumulative gas production (ml) up to 24 hr was higher in T2 (71.17), T3(69.77) T4(69.83) andT5(73.17) followed by T1(68.83). T6(64.50),T7 (64.03) and control (50.90). The methane production ( percent of total gas) in 18hr and up to24hr of incubation was recorded higher in all the probiotics treated groups except T3 group over control. Total VFA production increased in all treatments except T7 than control. Acetate proportion was reduced significantly in all the treatments with lowest value in T3(49.44) and highest in control (70.16). Significantly higher propionate and butyrate concentration was observed in all the treated groups over control. Reduction of ammonia-N was observed in all the trentments over control. In the viable culture added bottles total bacterial counts were increased significantly over control. Total protozoal population was statistically similar among all the treatments. Double and triple culture groups were mainly represented by yeast(60%), hence, variation among treatment groups was not so evident on most of the parameters estimated. But these cultures, most specifically yeast (S.cerevisiae, both strain), have the potential to improve in vitro rumen fermentation pattern.