Reconstruction of abdominal wall defects with glutaraldehyde treated xenogenic acellular dermal graft in rabbits: Gross, histopathological and histochemical studies

Abstract

Clinically healthy New Zealand White rabbils (12) of either sex were divided into group 1 (8) and 2 (4). Under thiopental sodium (2.5%) anaesthesia a full thickness abdominal wall defect (2cm-3cm) was created in group 1 and repaired with glutaraldehyde treated acellular dermal graft (G-ADG), whereas in group 2 linear abdominal incision was repaired with nylon suture. Increased vascularity and band like adhesions with caecum and colon were observed in group 1 on gro,ss examination, whereas, no adhesions were seen in control group. Histpathalogically. fibrous connective tissue invasion into the graft to variable distances and at day 60 tight junctions of fibrous tissue between host and graft was seen. Histochemically. alkaline phosphate activity increased upto day 30 in group 1 whereas, moderate intensity in control group was seen on day 14.