Avian chorioallantoic membrane-an in-ovo model for detection of anti-coccidial drug resistance

Abstract

An anticoccidial sensitivity test on the lines of antibiotic sensitivity test using embryonated eggs was standardized in the present study to detect the resistance. The sensitivity of the anticoccidial drug was tested by observing the ability of developmental stages of Eimeria species obtained from field isolates to establish and invade the interstitial cells of the chorio-allanloic membrane (CAM) of the embryonated eggs in the presence and absence of anticoccidial drug. Several discreet haemorrhagic foci were observed in CAM in less sensitive, resistant anticoccidial drug while no such foci was seen in sensitive anticoccidial drug interfering with the development of sporozoites the interstitial cells of the CAM.