Effect of plane of nutrition on Haematological and Blood Biochemical profile of pregnant Murrah buffaloes

Abstract

This research assessed the impact of enhanced metabolizable energy (ME) and metabolizable protein (MP) supplementation in the final trimester of pregnancy on hematological and biochemical parameters in Murrah buffaloes. Forty pregnant buffaloes were allocated into four groups, each receiving diets with varying levels of metabolizable energy (ME) and metabolizable protein (MP): control (100% ME and MP), HME (130% ME, 100% MP), HMP (100% ME, 140% MP), and HMEMP (130% ME, 140% MP). Monthly blood samples collected until parturition were analyzed for haematological indices, plasma glucose, total protein, albumin, blood urea nitrogen (BUN), creatinine, immunoglobulins, liver enzymes, and antioxidant status. The findings indicated that buffaloes receiving higher protein and energy diets (HMP and HMEMP) exhibited significantly enhanced red blood cell counts, hemoglobin concentration, packed cell volume, plasma glucose, total protein levels, BUN, immunoglobulin concentration, Cortisol and SOD antioxidant activity in comparison to the control group. Increased BUN levels in these groups indicate heightened protein metabolism. No notable alterations were detected in leukocyte profiles. The findings indicate that increased dietary ME and MP in late pregnancy positively affect maternal physiological and overall health status, potentially enhancing fetal development and the postnatal health of calves. This nutritional strategy may enhance health, welfare and reduce stress in buffaloes.