SNP identification in β2M gene and its association with IgG concentration in newborn buffalo calves

Abstract

β₂M (beta 2 microglobulin) gene is a non-covantely associated protein of MHC class I molecules found on the surface of nearly all cells. The present study was under taken to find out allelic variants in β₂M gene in Murrah buffalo and their association with serum IgG concentration of newborn buffalo calves. Twenty three newly parturated Murrah buffaloes and their respective new born calves maintained at Instructional Dairy Farm (IDF), G. B. Pant University of Agriculture and Technology, Pantnagar, were included in the present study. Polymerase chain reaction- restriction fragment length polymorphism (PCR-RFLP) technique was used to explore the polymorphism in β2M gene. The sequencing of different patterns obtained by RFLP of various exonic fragments revealed one SNP. The identified SNP was used to prepare the nucleotype for the calves. Indirect ELISA was done to estimate IgG concentrations in serum samples of the calves. IgG levels in calf serum ranged from 4.2 to 14.88 mg/ml and mean IgG concentration in serum was estimated at 11.23 ± 0.70 mg/ml. The least square analysis of variance exhibit a non-significant effect of calf nucleotype on serum IgG concentration.