Detection of palm oil adulteration in ghee using gas chromatography
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Keywords:
Adulteration, Butyric acid, Ghee, Gas liquid Chromatography, Linoleic acid, Palm oilAbstract
Ghee, is clarified butter or anhydrous milk fat, has an integral relation with Indian culinary culture. Due to the importance and seasonal decline in the production; unscrupulous traders tend to add cheap vegetable oils like palm oil in ghee. The composition of milk fat and oil vary in terms of fatty acids. Milk contains mainly short and medium chain fatty acids whereas vegetable oil contains long chain fatty acids. Due to different fatty acids composition between milk fat and palm oil, we tried to exploit this fatty acids difference to identify the adulteration of palm oil in ghee, using Gas chromatography. Study suggested that due to palm oil adulteration in ghee, the concentration of short chain fatty acids get decreased and long chain saturated and unsaturated fatty acids showed an increase. In addition to the above, change was also observed in the ratio of the total of saturated fatty acids to unsaturated fatty acids. Fatty acids analysis could be very effective for the detection of palm oil adulteration in ghee to the tune of 5 %. Our current study suggested that fatty acids study was able to detect foreign fat like palm oil in milk fat but this study is not suitable to confirm the type of foreign fat in milk fat or ghee.
References
Aparnathi KD, Sharma S, Antony B, Mehta BM (2019) Development of method for detection and quantification of foreign oils and fats in ghee (heat clarified milk fat) using FT NIR spectroscopy coupled with chemometric. Indian J Dairy Sci 72: 12-22
Bector BS, Sharma V (2002) Physico-chemical characteristics of ghee adulterated with palm oil and its detection. NDRI Annual report, pp. 44-45.
Bharwade M, Balakrishnan S, Chaudhary N, Jain AK (2017) Fatty acid profile and physico-chemical characteristics of milk lipids of Kankrej cow. Inter J Curr Microbiol Appl Sci 6: 3035-3047
De S (2011) Outlines of Dairy Technology. Oxford University Press, New Delhi
DeMan JM (1964) Determination of the fatty acid composition of milk fat by dual colomn temperature programmed GLC. J Dairy Sci 47: 546-547
GAIN (2014) India Dairy and Products annual. Gain Report No: IN 4089, Recent GAIN Publications. https://gain.fas.usda.gov Accessed 23 August 2019
Kumar A, Upadhyay N, Gandhi K, Naik SN, Sharma V (2017) Detection of adulteration in anhydrous milk fat (ghee) using season variation in Butyro-refractometer reading studied by employing dry fractionation technique. Indian J Dairy Sci 70: 563-570
Kumar A, Upadhyay N, Padghan P, Gandhi K, Sharma V (2015) Detection of vegetable oil and animal depot fat adulteration in anhydrous milk fat (ghee) using fatty acid composition. MOJ Food Process Technol 1: 13-19
Luddy RE, Herb SF, Magidman P (1968) A rapid method and quantitative procedure for the preparation of methyl esters of butter oil and other fats. J Am Oil Chem Soc 45: 549-552
Mehta BM, Antony B, Sharma S, Ratnam K, Aparnathi KD (2018) Comparative appraisal of FT MIR spectra of ghee (heat clarified milk fat) with that of the mutton tallow and vegetable fat. Indian J Dairy Sci 71: 240-245
Mehta M (2013) Consumption pattern and fatty acid composition of ghee. Food Sci Research J 4: 116-120
Orsavova J , Misurcova L, Ambrozova VJ, Vicha R, Mlcek J (2015) Fatty Acids Composition of Vegetable Oils and Its Contribution to Dietary Energy Intake and Dependence of Cardiovascular Mortality on Dietary Intake of Fatty Acids. Int J Mol Sci 16: 12871-12890
Pathania P, Sharma V, Arora S, Rao PS (2020) A novel approach to detect highly manipulated fat adulterant as Reichert–Meissl value-adjuster in ghee (clarified butter) through signature peaks by gas chromatography of triglycerides. J Food Sci Technol 57: 1-9
Ramani A, Hazra T, Parmar MP, Sindhav RG, Ramani VM (2019) Simple rapid technique for detection of palm oil in ghee. Indian J Dairy Sci 72: 441-444
Rebechi SR, VElez MA, Vaira S, Perotti MC (2015) Adulteration of Argentinean milk fats with animal fats: detection by fatty acids analysis and multivariate regression techniques. Food Chemistry. doi.org/10.1016/j.foodchem.2015.07.107
Upadhyay N, Kumar A, Lal D, Kant R, Goyal A (2018) Detection of groundnut oil and goat body fat adulteration in ghee using principal component analysis on fatty acid profile. Indian J Dairy Sci 71: 464-472