A Study on Detection of Soybean Oil and Buffalo Depot Fat in Ghee Using Normal-Phase Thin Layer Chromatography
344
Authors
-
Anil Kumar
PhD scholar, National Dairy Research Institute, Karnal, Haryana-132001
-
Neelam Upadhyay
PhD scholar, National Dairy Research Institute, Karnal, Haryana-132001
-
Kamal Gandhi
PhD scholar, National Dairy Research Institute, Karnal, Haryana-132001
-
Darshan Lal
Principal Scientist & Head,
Division of Dairy Chemistry, National Dairy Research Institute, Karnal (Haryana), India.
-
Vivek Sharma
Senior Scientist
Division of Dairy Chemistry, National Dairy Research Institute, Karnal (Haryana), India.
Keywords:
Thin layer chromatography, Unsaponifiable matter, Adulteration, Ghee, Soybean oil, Buffalo depot fat.Abstract
A study was carried out to detect soybean oil and buffalo depot fat, when adulterated individually in cow and buffalo ghee @5, 10, 15% (v/v) respectively, by using normal phase thin layer chromatography (TLC). Unsaponifiable matter (USM) of all the samples were extracted by using diethyl ether after saponification with alcoholic potassium hydroxide and were made alkali free by washing with water, followed by drying and dissolving in chloroform. Spotting of USM from all the samples was done on TLC plates, along with some reference standards of sterols (cholesterol, cholesterol acetate, β-carotene, phytosterols and tocopherols) and the plates were developed by using suitable solvent system (cyclohexane: ethyl acetate: water::600:200:1, v/v). It was observed from the results that soybean oil was easily detected even at the level of 5%, in both types of the ghee studied, by getting three extra spots or bands (Rf values 0.465, 0.512, 0.557) in the reference area as compared to pure samples, while buffalo depot fat didn’t show any additional marks in comparison to pure samples of cow and buffalo ghee.
