Reverse -phase thin layer chromatography of unsaponifiable matter extracted from ghee added with soybean oil and buffalo depot fat separately with a view to detect adulteration
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Authors
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Anil Kumar
Research Scholar, National Dairy Research Institute, Karnal, Haryana
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Neelam Upadhyay
Research Scholar, National Dairy Research Institute, Karnal, Haryana
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Amit Kumar
M.Tech. Scholar, National Dairy Research Institute, Karnal, Haryana
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Darshan Lal
Principal Scientist, National Dairy Research Institute, Karnal, Haryana
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Vivek Sharma
Senior Scientist, National Dairy Research Institute, Karnal, Haryana
Keywords:
Reverse phase thin layer chromatography, Unsaponifiable matter, Adulteration, Ghee, Soybean oil, Buffalo depot fat.Abstract
Reverse phase-thin layer chromatography was used in study to detect soybean oil and buffalo depot fat which were added individually in cow and buffalo ghee @ 5, 10 and 15% (v/v) levels, respectively. The unsaponifiable matter (USM) of all the samples were extracted after saponification and used for spotting on TLC plates, along with some reference standards of sterols (cholesterol, cholesterol acetate, ergosterol, β-sitosterol, β-carotene and mixture of α-, γ- and δ-tocopherols) and the plates were developed by using suitable solvent system (petrol ether: acetonitrile: methanol::2:4:4, v/v). It was observed from the results that soybean oil was easily detected even at the level of 5%, in both types of ghee studied, by getting two extra bands (Rf values 0.259 and 0.288) in the reference area as compared to pure samples, while buffalo depot fat showed the same pattern as that of pure ghee samples and hence remained undetectable.
