Effect of oral dosing of live and autoclaved culture of Ruminococcus flavefaciens FD-1 on rumen bacterial and fungal populations in Murrah buffaloes

Abstract

Dietary interventions aiming at increasing the number and activities of beneficial gut microbes could enhance digestive functions as well as the long-term welfare of dairy animals. In the present experiment, the effect of supplementation of a novel bacterial culture Ruminococcus flavefaciens FD-1 isolated from the rumen liquor of Murrah buffaloes was studied for its effect on rumen microbial populations enumerated using the most probable number technique. Three permanently fistulated buffaloes maintained on a high fibre diet were used as the source of rumen fluid for bacterial isolation that was subsequently tested
in vivo. Twelve healthy mid-to-late lactating buffaloes in their second to third parity were divided into two similar groups of six each with a mean body weight of 601.5 kg. One group (LBC) was dosed with 300 mL of live bacterial culture, whereas an equal volume of autoclaved bacterial culture was dosed in another
group (ABC) for a period of one month. The treatment-wise effect of supplementation of R. flavefaciens FD-1 culture was evident in an increase (P<0.05) in total rumen fungal population by 2.7 times in LBC than ABC in the post-dosing period. However, the period-wise comparison revealed that the population of bacteria augmented (P<0.05) by 2.16 and 3.17 times in both the groups ABC and LBC, respectively; whereas the magnitude of increase
in fungal population was 5.04 and 10.3 times, respectively for both the groups. Post-dosing effects were evident for only group LBC in both bacteria and fungi, respectively increasing (P<0.05) the population by 2.16 and 8.28 times than pre-dosing period. These preliminary results may foster scope for developing
species-specific (autochthonous) bacterial probiotic/direct-fed microbial based on R. flavefaciens FD-1 for large ruminants maintained on fibrous diets under tropical production systems.