Exopolysaccharide production potential of different strains of Lactobacillus plantarum
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Authors
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Riya KB
College of Veterinary and Animal sciences, Mannuthy,
Thrissur, Kerala
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K Radha
College of Veterinary and Animal sciences, Mannuthy,
Thrissur, Kerala
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C T Sathian
College of Veterinary and Animal sciences, Mannuthy,
Thrissur, Kerala
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MV Chinnu
Department of Bio chemistry,
College of Veterinary and Animal sciences, Mannuthy,
Thrissur, Kerala
Keywords:
Carbon and nitrogen sources, Exopolysaccharide, Lb. plantarum, Functional groups, Prebiotic activityAbstract
In the present study, the exopolysaccharide production potential of different strains of Lb. plantarum was investigated. Six different strains of Lb. plantarum were screened for their ability to produce EPS and the growth conditions were optimized for maximum EPS production. For this different strains of Lactobacillus plantarum were grown on MRS broth supplemented with carbon sources (glucose, sucrose) and nitrogen sources (yeast extract and beef extract) at three different levels for maximum EPS production. Sucrose (15g\L) was found to be the best source of carbon for maximum EPS production in strains NCDC 373 (50.87±7.67 mg/L) and NCDC 377 (54.50±1.27 mg\L). In NCDC 382 and UBLP-40, maximum EPS yield (88.48±3.55 and 84.41±3.24 mg\L) were obtained with 25g\L of sucrose. Beef extract acted as the best source of nitrogen for maximum EPS production in five strains (NCDC 378, NCDC 377, NCDC 382, UBLP-40, NCDC 20). FT-IR spectral analysis and prebiotic activity score of the purified EPS of Lb. plantarum strains NCDC 382 and UBLP-40 revealed the presence of prominent polysaccharide functional groups and their prebiotic potential respectively.Â
References
1. Adebayo-Tayo BC, Onilude A (2008) Screening of LAB strains isolated from some Nigerian fermented foods for exopolysaccharide production. World Appl Sci J 4: 714-747
2. BajpaiVK, Majumder R, Rather IA, Kim K (2016) Extraction, isolation and purification of EPS from LAB using ethanol precipitation method. Bangladesh J Pharmacol 11: 573-576
3. Braissant O, Decho AW, Pizekop KM, Gallagher KL, Glunk C, Dupraz C, Visscher PT (2009) Characterization and turnover of exopolymeric substances in a hypersaline microbial mat. FEMS Microbiol Ecol 67: 293-307
4. Dubiois M, Gilles JK, Hamilton PA, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28: 350-356
5. Fan L, Soccol AT, Pandey A, Sosscol CR (2007) Effect of nutritional and environmental conditions on the production of exopolysaccharide of Agaricus brasilliensis by submerged fermentation and its antitumour activity. Food Sci Technol 40: 30-35
6. Goh KKT, Haisman DR, Archer RH, Singh H (2005) Evaluation and modification of existing methods for the quantification of exopolysaccharide in milk based media. Food Res Int 38: 605-613
7. Grosu-Tudor S, Zamfir M (2013) Prebiotic potential of some EPS produced by lactic acid bacteria. Romanian Biotechnol Lett 18: 26-31
8. Hongpattarakare T, Chemtong N, Wichienchot S, Kolida S, Rastall RA (2012) In vitro prebiotic evaluation of EPS produced by marine isolated LAB. Carbohydr Polym 87: 846-852
9. Imran MYM, Reehan N, Jayaraj KA, Ahamed AA, Dhanasekaran D, Thajuddin N, Alharbic NS, Muralitharan G (2016) Statistical optimization of EPS production by Lactobacillus plantarum NTM105 and NTM120. Int J Biol Macromol 83: 731-745
10. Ismail B, Nampoothiri KM (2010) Production, purification and structural characterization of an EPS produced by Lb. plantarum MTCC 9510. Arc. Microbiol 192: 1029-1057
11. Mostefaoui A, Hakem A, Yabrir B, Boutaiba S, Badis A (2014) Screening for exopolysaccharide - producing strains of thermophilic LAB isolated from Algerian raw camel milk. African J Microbiol Res 8: 2208-2214
12. Rimada PS, Abraham AG (2003) Comparative study of different methodologies to determine the EPS produced by Kefir grains in milk and whey. Le Lait 89: 79-87
13. Shao L, Wu Z, Zhang H, Chen W, Aie L, Guo B (2014) Partial characterization and immunostimulatory activity of EPS from Lb .rhamnosus KF5. Carbohydr Polym 107: 51-56
14. Tallon R, Bresollier P, Urdaci MC (2003) Isolation and characterization of two exopolysaccharide produced by Lactobacillus planatrum EP56. Res Microbiol 154: 705-712
15. Tsuda H, Miyamoto T (2010) Production of EPS by Lb.plantarum and the prebiotic activity of the exopolysaccharide. Food Sci Technol Res 16: 87-92
16. WangY, Li C, Liu P, Ahmed Z, Xiao P, Bai X (2010) Physical characterization of exopolysaccharide produced by Lactobacillus plantarum KF5 isolated from Tibet Kefir. Carbohydr Polym 82: 895-903
