Exopolysaccharide production potential of different strains of Lactobacillus plantarum
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Authors
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Riya KB
College of Veterinary and Animal sciences, Mannuthy,
Thrissur, Kerala
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K Radha
College of Veterinary and Animal sciences, Mannuthy,
Thrissur, Kerala
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C T Sathian
College of Veterinary and Animal sciences, Mannuthy,
Thrissur, Kerala
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MV Chinnu
Department of Bio chemistry,
College of Veterinary and Animal sciences, Mannuthy,
Thrissur, Kerala
Keywords:
Carbon and nitrogen sources, Exopolysaccharide, Lb. plantarum, Functional groups, Prebiotic activityAbstract
In the present study, the exopolysaccharide production potential of different strains of Lb. plantarum was investigated. Six different strains of Lb. plantarum were screened for their ability to produce EPS and the growth conditions were optimized for maximum EPS production. For this different strains of Lactobacillus plantarum were grown on MRS broth supplemented with carbon sources (glucose, sucrose) and nitrogen sources (yeast extract and beef extract) at three different levels for maximum EPS production. Sucrose (15g\L) was found to be the best source of carbon for maximum EPS production in strains NCDC 373 (50.87±7.67 mg/L) and NCDC 377 (54.50±1.27 mg\L). In NCDC 382 and UBLP-40, maximum EPS yield (88.48±3.55 and 84.41±3.24 mg\L) were obtained with 25g\L of sucrose. Beef extract acted as the best source of nitrogen for maximum EPS production in five strains (NCDC 378, NCDC 377, NCDC 382, UBLP-40, NCDC 20). FT-IR spectral analysis and prebiotic activity score of the purified EPS of Lb. plantarum strains NCDC 382 and UBLP-40 revealed the presence of prominent polysaccharide functional groups and their prebiotic potential respectively.Â
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