Isolation and characterization of oleaginous yeasts from dairy waste

Abstract

Microbial lipids (biodiesel) can be produced by oleaginous yeasts via converting carbohydrates into lipids under certain conditions. Sudan black ‘B’ staining technique was used to screen cellular lipid accumulation. Extraction of the bacterial lipids was carried out by Bligh and Dyer method and fatty acid methyl esters were analysed by GC. Primary screening of sample resulted in 22 isolates, out of which 6 isolates were found to generate lipid. After detection of good oleaginous lipid productivity, one best yielding isolate was preferred for upward spring. Secondary selection of potential isolate was based on the production in addition to parameters like pH and nutritional requirements. Lipid from selected isolate CNK 1 was subjected to determination of saponification value and PUFA screening. The isolate CNK1 was identified as Zygrosaccharomyces rouxii by FAME analysis. The above results were promising hence it has importance for supplementary development of the yeast isolate Zygrosaccharomyces rouxii as another source of lipid for biodiesel production.