Prevalence of Enterococcus faecalis and Pseudomonas aeruginosa from mastitis milk and their antimicrobial resistance

Antimicrobial resistance pattern of Enterococcus faecalis and Pseudomonas aeruginosa isolated from mastitis milk

Abstract

Mastitis an inflammatory condition of mammary glands is a multi-factorial disease of dairy animals. It is characterized by physical, chemical and bacteriological changes in the milk. In the present study prevalence of Enterococcus faecalis (E. faecalis) and Pseudomonas aeruginosa (P. aeruginosa) were studied along with their antibiotic resistance pattern. One hundred and ten milk samples were screened from mastitis cattle and buffaloes in and around Ludhiana from December 2020 till June 2021. The prevalence of E. faecalis and P. aeruginosa was 7.05% and 4.70% respectively in mastitis milk samples tested. The isolates of E. faecalis were resistant to penicillin G (100%), vancomycin, erythromycin, and tetracycline (83.33%) and sensitive to nalidixic acid (100%), streptomycin (83.33%), oxacillin, gentamicin, ciprofloxacin and ceftriaxone (66.66%) whereas isolates of P. aeruginosa were resistant to ampicillin, ciprofloxacin, ceftriaxone, penicillin G, streptomycin, amikacin, vancomycin (100%), tetracycline and teicoplanin (75%) and sensitive to cefuroxime, gentamicin, and oxacillin (100%). In E. faecalis, vanA, vanB (50%), tetL (83.33%) and mrsA/B (100%) antibiotic genes could be amplified whereas in P. aeruginosa, aadA, DHAM (100%), sulI, sulII (100%), gyrA, gyrB (100%) and tetC (50%) antibiotic genes were amplified. Thus, it is concluded that there is an evolving prevalence of environmental pathogens such as E. faecalis and P. aeruginosa in mastitis which is alarming and thus necessary action and plans should be followed for controlling the antibiotic resistance in these pathogens as these are of zoonotic significance