THE Performance evaluation of strip based test for detection of urea adulteration in milk
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Authors
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A I Shaikh
A. I. Shaikh, Associate Professor, Dairy Chemistry Department, SMC College of Dairy Science, Anand
https://orcid.org/0000-0002-8094-2799
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Ravi Savaliya
Junior Officer, Banas Dairy, Palanpur, Gujarat
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Sonali Parekh
Research Associate, SMC College of Dairy Science, Kamdhenu University, Anand, Gujarat, India
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Daharti Kapadiya
Senior Research Fellow, SMC College of Dairy Science, Kamdhenu University, Anand, Gujarat, India.
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AK Jain
Assistant Professor, SMC College of Dairy Science, Kamdhenu University, Anand, Gujarat, India.
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Satish Parmar
Abstract
Milk samples spiked with various concentrations of urea ranging from 0.02, 0.04, 0.06 and 0.08% was used to check the urea detection ability of the developed strip. The LoD in each type of milk viz. raw, pasteurized and boiled was found to be 0.07% urea. Further, 121 (nos.) milk samples (cow, buffalo and mixed milk) were analyzed with the MilkoScan FT120 and developed strip for urea content. The urea content of the milk sample obtained using MilkoScan was compared with the colorimetric result of the strips. Both these results were in alignment. Moreover, the data of urea content obtained from MilkoScan and strip were used to calculate sensitivity, specificity and overall accuracy of the strip which were found to be 90.90, 92.00 and 90.09%, respectively. The strip performance was also checked for its effectiveness spectrophotometrically using 10 mixed milk samples. The developed strips were found to be stable when stored at room temperature (30-40°C) and refrigeration temperature (7±2°C) up to six months.
