Effect of fortification of commercial glycerol and crude glycerol to straw based mixed substrate on fermentation kinetics and energy value
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Authors
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G Savitha
National Dairy Research Institute
Southern Regional Station
Adugodi, Bengaluru - 560030
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E Lokesha
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Thingujam Suson
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S N Sondur
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Bandla Srinivas
Keywords:
Crude glycerol, fermentation kinetics, in vitro, pure glycerol, substrate utilizationAbstract
The study evaluated the effect of commercial grade pure glycerol (PG) and, crude glycerol (CG) which was obtained during biodiesel production on rumen fermentation kinetics in vitro. Glycerol purity of both the sources was 99.9% and confirmed with GC-MS. CG was little alkaline with 7.11 pH and consisted total tannins, alkaloids and flavonoids 24.09±3.21, 16.63±3.97 and 20.52±4.11µg/ml, respectively than PG. In vitro fermentation kinetics (IVFK) and substrate utilization by rumen microbes was studied by applying first order kinetics to in vitro gas production (IVGP) technique. Substrate having roughage to concentrate ratio of 60:40 was used without glycerol fortification (control) or fortified with10% PG or CG. Total gas production (TGP) was significantly (P<0.01) increased in PG or CG compared to control. Lag time in CG was more (P<0.05) than PG due to presence of impurities. Substrate IVDMD and IVNDFD were comparable between PG and CG. IVTVFA was significantly (P<0.01) increased in PG or CG than control. Metabolizable energy (ME) value of the substrate was improved by 27 and 32% respectively, in PG or CG compared to control. Study concluded that IVFK and substrate utilization between commercial or crude glycerol was comparable. Based on improved TVFA concentration and ME value, CG or PG could be fortified to dairy ration to enhance substrate fermentation and energy yield.
References
AOAC. 2012. Official Methods of Analysis. 19th edn. Association of Official Analytical Chemists.Washington. USA.
Avila, JS, Chaves, AV, Hernandez-Calva, M, Beauchemin, KA, McGinn, SM, Wang, Y, Harstad, OM. and McAllister, TA. 2011. Effects of replacing barley grain in feedlot diets with increasing levels of glycerol on in vitro fermentation and methane production. Animal Feed Science andTechnology.66:265–268.
Barnett, JGA. and Reid, RL. 1956. Studies on production of volatile fatty acids from the grass by rumen liquor in an artificial rumen. Journal of Agricultural Science.48: 315
Benedeti, DB, Silva, GP, Paula, ML, Shenkoru, E, Marcondes, T, Monteiro, MI, Amorati, HF, Yeh, B, Poulson, Y. andFaciola, SR. 2015. Effects of Partial Replacement of Corn with Glycerin on Ruminal Fermentation in a Dual-Flow Continuous Culture System. PLoS One 10: e0143201.
Blummel, M, Makkar, HPS. and Becker, K. 1997. In vitro gas production: a technique revisited. Journal of Animal Physiology and Animal Nutrition. 77: 24–34.
Chanjula, P, Pakdeechanuan, P, Wattanasit, S. 2014. Effects of dietary crude glycerin supplementation on nutrient digestibility, ruminal fermentation, blood metabolites, and nitrogen balance of goats. Asian Australasian Journal of Animal Science. 27: 365–374.
Das, M.N. and Giri, N.C. 1991. Design and Analysis of Experiments. Wiley Eastern Ltd., NewDelhi, India.
Dikshit,AK. andBirthal, PS. 2010. India’s Livestock Feed Demand: Estimates and Projections. Agricultural Economics Research Review.23: 15-28.
Donkin, S. 2008. Glycerol from Biodiesel Production: The New Corn for Dairy Cattle. Research BrazilizZootechnology. 37: 280-286.
EFSA. 2010. Panel on Contaminants in the Food Chain (CONTAM) Scientific Opinion on the abiotic risks for public and animal health of glycerine as co-product from the biodiesel production from Category 1 animal by-products (ABP) and vegetable oils. EFSA 12: 1934-1945.
Fisher, LJ, Erfle, JD. and Sauer, FD. 1971. Preliminary evaluation of the addition of glucogenicmaterials to the rations of lactating cows. Canadian Journal of Animal Science. 25:721-727.
France, J, Dijkstra, J, Dhanoa, M.S, Theodorou, MK, Lister, SJ, Davies, DR. and Isac, DA. 1993. A model to interpret gas accumulation profile associated with in vitro degradation of ruminant feeds. Journal of Theoretical Biology. 163: 99-111.
Lee W H, Intan S I. 2012. Antioxidant activity, total phenolics and total flavonoids of Syzygiumpolyanthum (Wight) walp leaves. International Journal of Medicinal and Aromatic Plants. 2: 219-228
Lokesha, E, Dhinesh, KR. and Srinivas, B. 2017. Effect of feed additives on in vitro fermentation kineticsand substrate utilization with and without buffer. Applied Biological Research 1: 56-62.
Mythili, K., Umamaheswara Reddy, C., Chamundeeswari, D. and Manna, P. K. 2014. Determination of Total Phenol, Alkaloid, Flavonoid and Tannin in Different Extracts of Calanthe Triplicata. Research and Reviews: Journal of Pharmocognsoy and Phytochemistry 2: 40-44.
National Biofuel Policy. 2015. Ministry of New and Renewable Energy National Policy on Biofuels New Delhi. Available on www.teriin.org/policybrief/docs/biofuel.pdf access on 29th April, 2017.
Oskoueian, E., Abdullah, N. and Oskoueian, A. 2013. Effects of flavonoids on rumen fermentation activity, methane production and microbial population. BioMed Research International. Article ID 349129.
Posada, JA, Luis, E, Rincón, Carlos, A. and Cardona. 2012. Design and analysis of biorefineries based on raw glycerol: Addressing the glycerol problem. Bioresource Technology. 111:282–293.
REN21. 2015. Renewable Energy Policy Network for the 21st Century. Renewables 2015 Global status report.
Trabue, S, Scoggin, K, Tjandrakusuma, S, Rasmussen, MA. and Reilly, PJ. 2007. Ruminal fermentation of propylene glycol and glycerol. Journal of Agricultural and Food Chemistry. 55: 7043–7051.
USDA. 2015. India Biofuels Annual 2015 Global Agricultural Information Network (GAIN) Report Number IN-5079, United States Department of Agriculture (USDA) Foreign Agricultural Service, New Delhi.
Wang, C, Lui, Q, Huo, WJ, Yang, WZ, Dong, KH, Huang, XY. andGuo. G. 2009. Effects of feeding glycerol on rumen fermentation, urinary excretion of purine derivatives and feed digestibility in steers. Livestock Science. 121: 15–20.
