Comparative study on the expression profile of Aquaporin5 (AQP5) gene in skin fibroblast cells of native goats enabling their water utilization efficiency
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Authors
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Femi Francis
ICAR-National Dairy Research Institute, Karnal
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Ashutosh Ludri
ICAR-National Dairy Research Institute, Karnal
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Thulasiraman Parkunan
Banaras Hindu University
http://orcid.org/0000-0003-0044-3445
Abstract
Erratic rainfall and climate change has resulted in the water crisis leading to deleterious effects in the agricultural sector especially on livestock population. In this scenario, native breeds adapted to sustain their life in water deficit areas without compromising their productivity come to play a role. Breeds native to arid and semiarid regions have developed genetic adaptations for the efficient water use by making alterations in osmoregulatory genes like Aquaporin (AQP). While most of the expression studies of AQP genes have been limited to humans, mice and rats. Hence, the present study was conducted on the native goat breeds- Barbari and Sirohi, to know the variations in the relative expression profile of AQP5 mRNA on goat skin fibroblasts during different temperature and osmotic concentration. The relative expression of AQP5 mRNA was decreased during low temperature in both the breeds. Whereas, the relative mRNA expression of AQP5 gene in Sirohi breed was found to be increased significantly (P<0.05) than Barbari at high temperature. The skin fibroblast cells of both the breeds exposed to hyperosmotic medium at 37°C showed increase in the relative expression of AQP5 mRNA and mRNA expression value of Barbari was found to be significantly higher (P<0.05) than Sirohi breed. The skin fibroblast cells of Sirohi and Barbari breed subjected to hyperosmotic medium at higher temperature demonstrated a decrease in the relative expression of AQP5 mRNA showed the potential of survival in both the breeds during extreme climatic conditions.
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